Chapter 7 - Rhizospheric microbiome: organization and bioinformatics studies

Abstract

The region between a plant's root system and the soil around it is known as the rhizosphere. The rhizosphere's microbiome, which is made up of all the microbes that live there, is a complex microbial ecosystem that supports the terrestrial biosphere. Archaea, bacteria, fungi, picoeukaryotes, and viruses are just a few of the microbial species that interact with their plant hosts directly in this intricate biological system. An integrated multiomics approach can be used to reveal the composition of the rhizospheric microbiome through 16S ribosomal amplicons and metagenomics. The functional characteristics of the microbiome through metatranscriptomics, metaproteomics, transcriptomics and proteomics, and the signaling network within the rhizosphere enable the complexity of the rhizosphere. A number of bioinformatics tools and softwares are available for the statistical analysis and visualizing omics data. Specific bioinformatic workflows to cover large eukaryotic genomes get around the resource constraints on genome databases is essential to understand the diversity of rhizosphere microbiome.