Abstract
The characterization of molecules using vibrational energy is a widely used technique. One such technique is Raman spectroscopy, in that the composition of a molecule is determined with the help of Raman scattering. In this technique, when the incident photon hits the molecule, it results in a shift to a lower or higher wavelength depending on the energy of the incident photon. Through this, it is possible to get structural information about the sample. Another variation of Raman spectroscopy is surface enhanced Raman spectroscopy, in which due to electromagnetic or chemical enhancement, the weak Raman signal that is commonly encountered in Raman scattering is amplified many-fold. A major advantage with these techniques is that they are nondestructive and require minimal or no sample preparation. Over the years, these techniques have found myriad applications in forensic science, especially in samples of biological origin such as human remains, fingerprints, and body fluids as well as other samples such as recreational drugs, paints, dyes, pigments, explosives, gunshot residue, fibers, and soil. In this chapter, we present a basic overview of the principle and theory of Raman spectroscopy as well as its instrumentation and applications in forensic science.
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More From: Handbook of Analytical Techniques for Forensic Samples
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