Abstract
Horticulture includes cultivation of fruits, vegetables, herbs, flowers, and non-food crops such as grass and ornamental trees and plants that forms an important sector of agribusiness. Genetic improvement of horticulture crops for enhanced yield and quality have received increased attention. With the advent of genome-editing technologies, it is becoming feasible to introduce mutations within the genome leading to improved agronomic value. Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-mediated genome editing has been utilized in many crop plants and demonstrated its potential impact. Base editing (using deaminase) and prime editing (using reverse transcriptase) are the state-of-the-art techniques of CRISPR-editing system that introduces precise base conversions. Edits can be introduced into the RNAs by using the RNA base editing system leaving the DNA unharmed. This chapter covers the strategies for base editing and prime editing and the studies demonstrating the application of this technology for horticulture crop improvement. With improved editing efficiency and reduced off-target effects this technology will significantly increase horticultural crop production and quality.
Published Version
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