Abstract
This chapter discusses electrophoresis that can be used for the separation of amino acid derivatives in protein sequence analysis. The approach to the separation of underivatized amino acids remains almost the same, apart from small variations, whether one is dealing with the “classical twenty” or with rarely occurring amino acids. Separation methods for amino acids are well established, and the preferred procedures today are chromatographic. The electrophoretic separations possess considerab1e importance as they can be used effectively for screening purposes. Equally, electrophoretic methods can help in the search for the rarer amino acids that arise during post-translational reactions in proteins or that occur as the result of diverse metabolic processes and disorders. Two-dimensional paper separations can run at considerably higher voltages. In chromatoelectrophoresis, it is necessary to avoid possible interferences from the electrophoretic buffers in the subsequent chromatographic step. The arrangement of the “three-dimensional” separation could be modified in such a way that in the first run the complex mixture of amino acids is separated by electrophoresis on a cellulose acetate plate, cut into two parts, one of which is subjected to another electrophoretic separation, while the other part of the plate is separated chromatographically.
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