Abstract
Fluorescent labelling is widely used as an indispensable tool in biology for the study of complex molecular interactions. Conventional downconversion fluorescence labels with ultraviolet (UV) or short wavelength excitation suffer from the presence of autofluorescence, low signal-to-noise ratio and incident photodamage to living organisms. This chapter focuses on upconverting fluorescent nanoparticles with excitation in near-infrared region. This has several advantages including very low autofluorescence, absence of photodamage to living organisms, high detection sensitivity and high light penetration depth.
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