Abstract
This chapter presents an example on the structure identification and relative quantitative determination of plant polyphenols using high-performance liquid chromatography coupled with photodiode array (PDA) detection and tandem mass spectrometry without the use of standard compounds. The relation of molecular structure and antioxidant efficiency by chemometric evaluation of primary data is also discussed. Beech (Fagus sylvatica L.) bark was chosen as the plant material, because it is an abundant forestry byproduct rich in polyphenols worthy for future utilization. Using chemometric evaluations, those compounds were identified, mostly to determine antioxidant properties, which could influence the utilization of extracts by increasing antioxidant activity, or play a special role in plant defense reactions through the plant antioxidant and stress response system. The measurement and evaluation methods presented can be used for other plant materials to track polyphenol antioxidant effects.
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