Abstract
Publisher Summary This chapter discusses chemical derivatization techniques using microcolumns. The use of chemical derivatization as a means of improving detectability of a compound has been widely employed in classical high performance liquid chromatography (HPLC) on wide bore columns but derivatization techniques used in conjunction with narrow bore packed columns or open-tubular capillary columns, have been investigated currently in greater detail. Two most useful methods of chemical derivatization are offline pre-column and the online post-column derivatization, the latter is considered to be more interesting from the theoretical as well as experimental point of view and thus more emphasis is given in the chapter to post-column techniques. From the study of the kinetics of the chemical reaction, the reaction time necessary to reach maximum derivative concentration is determined in the chapter. Because the pre-column technique involves chromatography of the modified sample, a considerable amount of time has to be spent to determine the mobile/stationary phase conditions needed to elude the derivatives from the column.
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