Abstract
This chapter highlights the fundamental parameter of isoelectric focusing (IEF). The conventional IEF is performed in an anticonvective medium, such as the slab gel. The advantages and disadvantages of gels, apply equally to IEF. In contrast to the usual gel format, the pore size of an IEF gel should be sufficiently large for reducing the impact of molecular sieving, which otherwise would lengthen the run time. The chapter also defines that detection in the slab gel is time consuming and semi-quantitative. The carrier ampholytes must be washed out of the gel to avoid reaction with the staining reagent. Small peptides are not detectable, because they are lost during the wash step. Gels are unnecessary in capillary isoelectric focusing (CIEF), because the ampholyte separation media is effectively supported and contained by the capillary walls. CIEF is performed in free solution.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
