Abstract
Yinchenhao Tang (YCHT), a well-known Chinese herbal formulae, was recorded in Shanghan Lun, which is a classic resource of traditional Chinese medicine (TCM) written by Zhongjing Zhang (150–215 AD) and was officially listed in the Chinese Pharmacopoeia. It consists of Artemisia annua L. (the monarch herb), Gardenia jasminoides Ellis (the minister herb), and Rheum palmatum L. (the assistant and servant herb) and has been used for more than a thousand years to treat jaundice and liver disorders. It possesses many effects, such as a treatment for cholestasis, liver fibrosis, hepatitis C, biliary cirrhosis, and cholestatic diseases. Chemical studies on the single herbs of YCHT have been conducted. Serum pharmacochemistry of TCM has been used for the screening and analysis of effective compounds in TCM formulae. In this work, a thorough review of a great deal of information of the constituents of rat plasma is undertaken, and to identify the potential active constituents in YCHT, an ultraperformance liquid chromatography mass spectrometry (UPLC-MS) detector and C18 column with a 1.7μm particle size was utilized. As a result, under the same UPLC conditions, the chromatographic data were recorded for only 20min; 45 peaks were found in vitro and 21 peaks in serum were detected after oral administration of the YCHT. The structure identification of 21 peaks found in serum was confirmed by using elemental analysis, UV, and MS and by comparing with standards. This study has been undertaken to investigate the chemical constituents and the metabolites in vivo, and to explore the absorbed bioactive components in YCHT.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
