Abstract
Publisher Summary The interaction between lipids and proteins in membranes is a two-way process, each component affecting the properties of the other. It has been known that the organizational and motional properties of lipids are influenced by the presence of proteins. Likewise, the function of membrane proteins is affected by the lipid environment. In microsomal membranes, it has been found that several enzymes, such as fatty acid desaturases, glucose-6-phosphatase, and UDP-glucuronyl transferase (UDPGT), have their activities modulated by in vivo or in vitro manipulation of lipid composition. This chapter focuses on the work done on UDPGT, an enzyme whose activity is strongly affected by the lipid environment. The effect of in vivo cholesterol incorporation, in vitro cholesterol incorporation and depletion, and changes in acyl chain degree of unsaturation in guinea pig liver microsomes have been analyzed from the point of view of (1) enzyme kinetics; (2) bulk lipid properties, by means of fluorescent probes; and (3) lipid-protein interactions, by means of spin label probes.
Published Version
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