Chapter 32 - Use of Coffee Husk Waste for Production of Biopesticides for Mosquito Control

Abstract

The use of coffee husks from agro-industries is highly economical for the industrial production of mosquito pathogenic bacilli, in terms of easy availability, cost-effectiveness, efficacy, environmental safety, and biowaste remediation. The mosquitocidal spore/crystal toxins produced from the experimental culture medium are equal to that of conventional medium. The protein profiles of Bacillus sphaericus and Bacillus thuringiensis subspecies israelensis spore/crystal toxins produced from coffee husk waste is similar to that of nutrient yeast extract mineral salt medium. The entomotoxicity studies with different mosquito species showed that the efficacy of B. sphaericus and B. thuringiensis subspecies israelensis toxins produced from experimental and conventional media were comparable. The raw material cost analysis indicated that the use of coffee husk waste is highly economical for the industrial production of these mosquito pathogenic bacilli. This study is, therefore, very important from the perspective of recycling of environmental waste because it possesses the dual benefits of effective use of environmental bio-organic waste and efficient production of mosquitocidal biopesticides.