Abstract
This chapter discusses the high-performance liquid chromatography (HPLC) of carbohydrates on graphitized carbon columns (GCCs). GCCs for HPLC are relatively new and are used for separations of samples such as pharmaceuticals, natural products, peptides, amino acids, simple aromatic molecules, and ionized and other polar compounds. Graphitized carbon behaves primarily as a stronger reversed-phase stationary phase than the alkyl-bonded silica gels. Hydrophobicity of GCCs is greater than that of other reversed-phase materials. Therefore, a greater percentage of organic modifier in the eluent is required, and GCCs are preferred to octadecylsilyl (ODS) columns for the separation of hydrophilic compounds. As GCCs are completely unaffected by strongly acidic or alkaline conditions and can be used throughout the pH range, separations with a much wider range of solvents become feasible. As the retention mechanism of GCCs is unique and GCC possess exceptional chemical and physical stability, they have a great potential for the separation and purification of isomeric and closely related carbohydrates.
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