Abstract
Fluorescent nanoparticle-based imaging probes are expected to generate new medical diagnostic tools because of their superior brightness and photostability. Although significant progress has been made regarding fluorescent semiconductor nanocrystal-based biological labeling, presence of heavy metals and their associated toxicity issues have severely limited their application potential. Fluorescent carbon nanoparticle appears as an alternative nontoxic imaging probe suitable for biological staining and diagnostics. The chemical synthesis of highly fluorescent carbon nanoparticles in the range of 1–10nm and with size-dependent tunable visible emission is significantly advanced. These carbon nanoparticles could be transformed into various functional nanoprobes of 5–15nm hydrodynamic diameters and could be used as a cell imaging probe. Stable and tunable visible emission, similar to semi-conductor nanocrystals, but small hydrodynamic size and low toxicity would offer wider application of these nanoprobes in biomedical science.
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