Abstract
Zinc-finger nucleases, transcription activator-like effector nucleases, and CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-guided endonucleases allow site-specific genomic editing. Each of these technologies has enormous therapeutic potential, and ongoing studies have shown their therapeutic potential in various diseases in both mammalian and human stem cell. These technologies use different methods to introduce a double-strand break at the target DNA site, and subsequent gene disruption or editing, depending on which repair pathway is undertaken. Here, we will review these three genetic engineering techniques, describing and comparing their function, efficacy, and use in recent studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: CRISPR Genome Surgery in Stem Cells and Disease Tissues
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
