Abstract

The enzymatic reaction is highly respected from an environmental-friendly aspect. Optimization of reaction media and supporting materials of enzymes must be investigated in parallel with the effort to develop new enzymes. Ionic liquids (ILs) have very good properties as reaction media in chemical reactions and even for biotransformation. ILs allowed significant improvement of the stability of enzymes by immobilization through a lyophilization process. In this chapter, we discuss the possibility of IL engineering focusing on lipase-catalyzed reactions and laccase-catalyzed reactions, that is, ILs mediated the enhanced reaction rate and improved the stability of enzymes.

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