Abstract
This chapter discusses some biological analytical methods. The chapter reviews some of the more common methods, and the instrumentation required. It discusses that a modern biochemical or respiratory function laboratory contains a considerable number of highly sophisticated and often automatic analytical instruments. Ionic composition of tissue fluids is reviewed in the chapter. Sodium and potassium, and chloride constitute the ionic composition. A typical concentration of sodium or potassium ion sprayed into the flame is about 0-5 mmol/l. Chloride estimations are commonly required in laboratory investigations, although they tend to be avoided in clinical biochemistry. Estimation of oxygen and carbon dioxide in biological solutions can be explained by oxygen in solution and carbon dioxide in solution. Oxygen concentration in solution is most simply estimated electrolytically. The ability of whole blood to transport carbon dioxide from the body tissues to the lungs is of vital importance, and an estimate of this function is frequently required.
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