Abstract
Plasma membranes change shape dynamically to exert numerous biological functions such as cellular morphogenesis, migration, attachment to other cells and substrates, and endo/exocytosis. However, only a few technologies capture the dynamics of fine membrane structures of few microns or less with the desired spatiotemporal resolution. Recently, a new technology using an ultrathin light-sheet that employs Bessel beam technology has been developed and used successfully to illuminate various life processes beyond the limitations of conventional technologies. This chapter introduces ultrathin plane illumination microscopy, which originated as Bessel beam microscopy and subsequently improved to lattice light-sheet microscopy, and examples visualizing membrane shape and dynamics.
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