Abstract
Tea polysaccharide conjugates (TPC) are protein-bound acidic polysaccharides. High-performance gel permeation chromatography (HPGPC)–evaporative light-scattering detection (ELSD) and circular dichroism (CD) were developed for the measurement of the thermal effects on the homogeneity and conformation of these TPC, respectively. Two water-soluble polysaccharide conjugate fractions were extracted from green tea by hot and alkaline water, respectively. Non-obese diabetic (NOD) mice were used to evaluate their antidiabetic bioactivities. Both fractions suppressed spontaneous diabetes mellitus in NOD mice. In other studies, crude TPC were extracted and purified to yield three hitherto unexplored polysaccharide-conjugate fractions, termed gTPC1, gTPC2 and gTPC3. The gTPC1–3, at three dose levels (50, 150 and 300 μg/mL), were found to possess remarkable dose-dependent protective effects on human, umbilical vein, endothelial (HUVE) cells against impairments induced by high glucose concentrations.
Published Version
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