Abstract
A variety of chromatographic modes have been developed on the basis of the mechanisms of retention and operation. The key chromatographic modes are normal-phase, reversed-phase, ion exchange, size-exclusion, and affinity chromatography. This chapter introduces the chromatographic modes and explains how they work. In addition to the major modes, there are a number of techniques that could be viewed as submodes. Examples of compounds separated by each mode, and the advantages and disadvantages, are provided to help chromatographers choose the most appropriate mode for a given group of analytes. There are a number of applications of high-performance liquid chromatography (HPLC). Normal-phase chromatography is used for the separation of neutral species on the basis of polarity, reversed-phase chromatography is used for the separation of neutral species on the basis of hydrophobicity, ion-exchange chromatography is used for the separation of ionic solutes on the basis of charge, size-exclusion chromatography is used for the separation of molecules on the basis of differences in molecular size, and affinity chromatography is used for the separation of biomolecules on the basis of the lock-and-key mechanism prevalent in biological systems.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: High Performance Liquid Chromatography & Capillary Electrophoresis
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
