Abstract

The chromatographic process is characterized by a number of parameters, some of which are related to the separation process and others to the detection/quantitation. This chapter describes the main parameters related to the control and description of the separation process. The parameters used for controlling the separation include descriptors of the mobile phase (e.g., flow rate) and descriptors of the stationary phase/chromatographic column (e.g., column dimensions, phase ratio Ψ, column efficiency, etc.). The separation process is described by parameters such as retention time and volume, capacity factor, selectivity, resolution, peak capacity, and peak asymmetry. Several interrelations between separation parameters are also discussed, such as the van Deemter equation.

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