Abstract
Flow cytometry covers a range of techniques that can be helpful to the neurobiologist in elucidating the characteristics and properties of neural cells. The objective of this introductory chapter is to support those new to the technology by providing them with the appropriate background information to design successful and meaningful experiments. This chapter introduces the fundamental concepts of flow cytometry and cell sorting. In particular, it explains in a stepwise fashion how a flow cytometer works and how an understanding of its operating principles is critical to generating meaningful reproducible results. Suggestions are provided for those new to the field on how to prepare samples and optimize cell sorting settings so that the instrumentation can provide the required results. Moreover, this chapter provides a brief overview of a selection of characteristic neural markers and options to label and identify specific neural cell subsets.
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