Abstract
Publisher Summary This chapter describes the isolation and characterization of nonhistone phosphoproteins. The nonhistone chromosomal proteins play a regulatory role in the control of gene expression. Two of the nonhistone protein (NHP) fractions (I and II) stimulate transcription in vitro , NHP-I by acting on chromatin and NHP-II by acting on DNA. The third fraction (NHP-III) inhibits transcription from DNA. The varied but specific effects of these nonhistone proteins indicate the involvement of nonhistone chromosomal proteins in both positive and negative controls in the differential regulation of gene expression. Nonhistone protein fractions restore histone-inhibited transcription from DNA. If NHP-I is added after DNA and histone have already been interacted, the restoration of histone-inhibited template activity of DNA for RNA synthesis is still effective however requires higher concentrations of the nonhistone proteins. The NHP-III is homogeneous when subjected to polyacrylamide gel electrophoresis.
Published Version
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