Abstract
Publisher Summary This chapter focuses on mutants of meiosis and ascopore formation. Formation of spores by both prokaryotic and eukaryotic microbes presents an opportunity to study the biochemical and genetic regulation of differentiation in single cells. Sporulation of yeasts is of particular interest, because it encompases meiosis and ascospore development. A comprehensive investigation of the genetic control of sporulation requires procedures that permit the detection of both recessive and dominant gene mutations. The detection of recessive mutations affecting meiosis and ascospore formation is complicated by the fact that these processes occur in diploid cells or cells of higher ploidy. Two systems have been developed that allow the recovery of both recessive and dominant mutations. One approach takes advantage of the life cycle of homothallic strains to obtain mutants in the homozygous condition in diploid cells. This procedure has been used with both Saccharomyces cerevisiae and Schizosaccharomyces pombe. Another technique employs certain n + 1 disomic strains of S. cerevisiae that begin but do not complete sporulation. In this chapter, detailed methods of isolation and characterization of mutants with defective sporulation are presented. The properties of the mutants and the information they have yielded regarding the control of sporulation functions are also discussed.
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