Abstract
The identification and quantification of polyphenols in food and biological samples is a difficult task due to the wide variety of polyphenol structures found in nature and the lack of commercially available standards. Several methods have been used, including spectrophotometric methods, capillary electrophoresis, nuclear magnetic resonance spectroscopy, near-infrared spectroscopy, and chromatographic techniques such as high- and ultra-high-performance liquid chromatography, high-speed counter-current chromatography, supercritical fluid chromatography, and gas chromatography. Clean polyphenol extracts are required before samples can be analyzed. Extraction methods include liquid–liquid extraction, solid–liquid extraction, extraction with supercritical fluid, and solid-phase extraction.
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