Abstract
The advent of genome synthesis in the 21st century opened up various approaches on cloning whole bacterial genomes. In particular, relevant technologies starting from chemically synthesized oligomers should enable genome reorganization not limited to existing genomes but expanded to any genomes with novel sequences designed even from scratch. Those cutting-edge technologies sooner or later will replace template-dependent gene cloning technologies, albeit technical difficulties regarding efficiency and cost preclude general use at present. Meanwhile, rationally designed genomes require thorough knowledge of existing genomes in retrospect. Certain unnoticed rules underlying functional genomes might be found through various kinds and levels of studies.
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