Abstract
Publisher Summary This chapter describes starch chain-length analysis using an anion-exchange chromatography system equipped with an enzyme reactor and a pulsed amperometric detector (PAD). Starch is the major source of energy in the diet of humans and animals. A starch granule is made up of amylose and amylopectin with trace amounts of other ingredients such as lipids and phosphate derivatives. With the knowledge of starch chemical structures and the effects of chemical structures on its functional properties and biosynthesis, starch can be genetically modified to induce a specific structure and achieve desired functional properties. The study of starch chain length is essential for understanding the chemical structure and biosynthesis of starch. Various techniques are adapted for starch chain-length analysis including high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC–PAD), gel permeation chromatography (GPC) with total carbohydrates and reducing end analyses, high-performance size-exclusion chromatography (HPSEC) with a refractive index (RI) detector , and HPSEC with an on-line laser light-scattering detector (LLS) and an RI (HPSEC–LLS–RI). All of these methods are widely used in the field and have their advantages and disadvantages in starch chain-length analysis.
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