Abstract
This chapter discusses the recent status of protein/enzyme engineering related to improvement activity, with an emphasis on the implications for industrial biotechnology. Different methods employed for enzyme engineering have been reviewed, such as directed evolution, rational design, site-directed mutagenesis, and semi-rational design. Information provided related to the mechanism for enhancement of enzyme activity is useful in industrial and environmental applications. Improvement in the yield of enzymes is difficult; however, increasing the activity of enzymes through various in silico approaches is useful. The high-throughput screening of various newly screened enzymes could further be tailored for use in prevalent industrial applications, with the help of enzyme engineering.
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