CHAPTER 12 - CHROMATOGRAPHY

Abstract

Publisher Summary This chapter describes chromatography technique. This technique has not only rendered easy many separations otherwise difficult but also has made possible many separations otherwise impossible, particularly in the fields of organic chemistry, biochemistry, and pharmacology. One of the simplest forms of chromatography is that known as elution analysis in which a suspension of a suitable adsorbent is prepared by putting a filter cone covered by a filter paper disc, a pad of cotton, or wool in the constricted end of the chromatographic tube, filling the tube one-third full of the solvent, adding a slurry of adsorbent and solvent, and allowing it to settle. Excess solvent is run off at the bottom and more adsorbent plus solvent added as necessary to make a column of the desired length. Solvent is run off until only a millimeter or so remains above the solid packing, and the solution of the sample added. The chromatogram is developed by allowing the mobile phase solvent to run through at a rate of 1 or 2 ml per minute.