Abstract
Since its introduction in 1975, two-dimensional electrophoresis (2-DE) has been the object of a number of improvements and adaptations. This progressive evolution witnesses the strong interest for this technology that moved from just a complicated generic analytical method to a modern and easy-to-make methodology in proteomics. Its contribution in this field is so extended that it became unavoidable when making plans for in-depth protein investigations. The performance of 2-DE is even increased when associated with enrichment technologies for the detection of very dilute proteins from crude extracts. It is the aim of this review to show this symbiosis with most recent developments in emerging applications such as the discovery of novel low-abundance allergens and pertinent biomarkers of clinical interest.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
