Abstract
Publisher Summary This chapter highlights use of micromanipulators in yeast studies. Micromanipulators are used in yeast genetic studies (1) for the separation of ascospores from individual asci, (2) for the separation of zygotes from mass-mating mixtures, (3) for positioning of vegetative cells and spores for mating purposes, and (4) for single-cell analyses. Micromanipulators usually can be subdivided into two major types: those having three separate controls for positioning the microtool and those having a single lever or joystick, which freely controls the microtool either in three dimensions of space or in one plane. Micromanipulators that operate with control levers or joysticks can translate hand movements into synchronously reduced movements of microtools. The Singer MKIII micromanipulator is a robust mechanical micromanipulator used for yeast genetic studies in several laboratories in England. In the Singer MKIII micromanipulator, lateral-hand movements control the horizontal movements of the microtool, while rotation of the same lever controls vertical movements.
Published Version
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