Abstract
High-throughput sequencing (HTS) of nucleic acids has revolutionized the biological sciences and ushered in new prospects for molecular medicine. Also referred to as next-generation sequencing (NGS) or massive(ly) parallel sequencing (MPS), HTS has in part superseded techniques such as Sanger sequencing and hybridization or amplification microarrays. As inputs into the HTS workflow, both DNA and RNA can be used, but the latter must be converted first into complementary DNA (cDNA). HTS for RNA (RNA sequencing or RNA-Seq) allows the analysis of the transcriptome at single-nucleotide resolution, identifying RNA sequences without a priori knowledge of genomic DNA, splicing, or posttranscriptional modifications. The technique can thus complement genomic DNA sequencing by capturing a “snapshot” of the transcriptomic output within an organism, tissue, cell, or even extracellular environment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Rigor and Reproducibility in Genetics and Genomics
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
