Chapter 10 - Analytical methods in the detection of proteins and peptides in tissue fluids and homogenates

Abstract

Analytical methods for the detection of proteins and peptides in tissue fluids and homogenates involve a variety of techniques, including mass spectrometry, enzyme-linked immunosorbent assay (ELISA), western blotting, and sodium dodecyl sulfate (SDS)-PAGE. These methods are used to identify and quantify specific proteins and peptides in biological samples and can provide important information about the presence and levels of these biomolecules in different tissues and fluids. Mass spectrometry is a highly sensitive and specific technique that can be used to identify and quantify proteins and peptides with high accuracy. ELISA and western blotting are commonly used to detect and quantify specific proteins and peptides in biological samples. SDS–PAGE is a widely used technique for the separation and analysis of proteins based on their size and charge. Together, these analytical methods provide a powerful toolkit for the detection and characterization of proteins and peptides in tissue fluids and homogenates and can be used to gain insights into the molecular mechanisms underlying a wide range of biological processes.