Abstract

Publisher Summary This chapter provides an overview of countercurrent chromatography—a liquid–liquid partition chromatography technique employing two immiscible liquid phases. One phase is designated as the stationary phase and is retained as such by either gravitational or centrifugal forces while the second phase, the mobile phase, is passed through the stationary phase. Several different types of instruments are currently available to perform countercurrent chromatography. The simplest form of continuous flow countercurrent chromatography is droplet countercurrent chromatography (DCCC). In rotary locular countercurrent chromatography (RLCC), columns are divided into separate chambers or loci along their length, with small centrally located perforation connecting adjacent segments. A series of these locular columns are aligned parallel to one another, connected by tubing and mounted on a rotatable disc. Spinning column countercurrent chromatography (SCCCC) consists of two concentric glass pipes with a 1 mm gap between them. This gap is filled with the stationary phase. The centrifugal countercurrent chromatography with rotary seal joint device (CCCC-RJ) employs a series of polytetrafluoroethylene (PTFE) resin blocks into which holes are drilled with connecting tubes added, creating 50 small “columns” per block.

Full Text

Published Version

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.