Abstract
Objective To build the rat animal model of neurogenic bladder induced by spinal cord injury and investigate the changes of urodynamics and P2X1 receptor expression in bladder detrusor after the spinal shock stage. Methods A total of 40 female Wistar rats were randomly allocated into control group, suprasacral injury group and sacral injury group. Complete spinal cord transection was established by cutting out of dura at T10-L2 in suprasacral injury group. The sacral injury group was done by cutting out of dura at S2-4. Urodynamic monitor was performed on rats after the spinal shock stage(6 weeks after operation). The P2X1 receptor expression in bladder detrusor among the 3 groups was checked by using immunohistochemical method. Results The suprasacral injury group exhibited detrusor hyperreflexia during bladder filling, elevated leak point pressure and compliance. The sacral injury group exhibited detrusor lower reflection, reduced leak point pressure and elevated compliance. The level of P2X1 receptor expression in rats' detrusor of suprasacral injury group was increased, and the level of sacral injury group was reduced. Conclusions Neurogenic bladder induced by spinal cord injury reveals significant changes in urodynamic after the spinal shock stage. The enhancement of the P2X1 receptor expression in detrusor may be one of the mechanisms of detrusor hyperreflexia. Key words: Spinal cord injury; Neurogenic bladder; Animal model; Urodynamics; P2X1 receptor
Published Version
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