Changes of transforming growth factor-beta 1 and p38 mitogen activated protein kinase and the effects of drug on rats after cardiopulmonary cerebral resuscitation

Abstract

Objective To observe the changes of transforming growth factor-beta 1 (TGF-β1) and p38 mito-gen activated protein kinase (p 38MAPK) , and the effects of Xuebijing on rats during cardiopulmonary cerebral re-suscitation. Method The animal model of cardiac arrest (CA) was made by clamping endotracheal tube at expi-ration, and it survived over 24 hours after cardiopulmonary resuscitation (CPR). Fifty healthy Wistar rats were randomly (random number) divided into control group, model group, small dose of Xuebijing group, medium dose of Xuebijing group and large dose of Xuebijing group. Those rats were sacrificed 24 hours later and the cortex of the brain and the heart were taken out immediately to observe the ultrastructure changes. The levels of TGF-β1 and p38MAPK were determined by using ELISA. Results Compared with control group, the ultrastructure of brain and cardiac tissues were damaged in all other groups, and the most severe damage was seen in the model group, and the leastamage was found in large dose of Xuebijing group. The levels of TGF-β1 in tissues of brain and heart of rats increased more significantly, while the levels of p38MAPK reduced more markedly in Xuebijing treated groups than those did in model group. The magnitudes of increase in TGF-β1 and decrease in p38MAPK were greater in medium dose of Xuebijing group and large dose of Xuebijing group than those in small dose of Xuebijing group. Conclusions The expressions of TGF-β1 and p38MAPK in brain and heart tissues of rats increase after cardiopulmonary cerebral resuscitation. The Xuebijing could significantly modulate the expressions of TGF-β1 and p38MAPK leading to lessening the damage of brain and heart in dose-dependent manner after CPR. Key words: Cardiac arrest(CA) transforming growth factor-beta 1; p38 mitogen activated protein kinase; Xuebijing