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Abstract
Proteomic analyses have already been used in a number of hepatological studies and provide important information. However, few reports have focused on changes in the cytoplasmic proteome. The present study therefore aimed to evaluate changes in cytoplasmic proteome of rats in response to alcoholic hepatotoxicity. Rats were fed a Liber-DeCarli liquid diet containing ethanol for four weeks. Cytoplasmic proteins except mitochondrial proteins from the livers of these animals were investigated using two-dimensional gel electrophoresis and mass spectrometry. Alcohol induced a decrease in body weight gain and an increase in alanine transaminase (ALT), cholesterol, and phospholipid levels. Histopathological observations revealed hepatic damage characterized by necrosis and fatty change in alcohol-treated group at week 2, which continues until week 4. Our proteomic analysis revealed that 25 proteins were differentially expressed in the ethanol-fed group. Of these, 12 cytoplasmic proteins are being reported for the first time. Taken together, our results provide further insights into the disease mechanism and therapeutic information of alcoholic liver disease.
Highlights
IntroductionAlcohol has cytotoxic effects on numerous tissues, the liver is one of the most common targets
Alcohol abuse is the leading cause of morbidity and mortality worldwide
Serum biochemical analyses showed that alcohol induced a significant increase in alanine transaminase (ALT), cholesterol, and phospholipid levels at 2 and 4 weeks after treatment
Summary
Alcohol has cytotoxic effects on numerous tissues, the liver is one of the most common targets. The stages of alcoholic liver disease (ALD) comprise steatosis, steatohepatitis, and fibrosis/cirrhosis [1]. The main causes of ALD were found to be malnutrition, redox changes by oxidation of ethanol, microsomal (especially CYP2E1) induction, formation of protein adducts from acetaldehyde and RNS/ROS formation. These result in antibody production, enzyme inactivation, decreased DNA repair, impaired utilization of oxygen, glutathione depletion, and increased collagen and ROS/RNS synthesis [2,3,4,5,6,7,8]
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