CHANGES OF MICROELEMENT COMPOSITION OF FEMUR AND TIBIA IN THE CONDITIONS OF DIABETIC ARTHROPATHY OF KNEE JOINT

Abstract

Joint damage in patients with diabetes is a fairly frequent complication. Features of the microelement composition of the femur and tibia and structural changes of the knee joint in diabetes have not been studied enough. The purpose of the study was to study the features of the microelement composition of the femur and tibia in diabetic arthropathy of the knee joint. The content of microelements (iron, copper, zinc, magnesium, manganese, lead) in the femur and tibia of 75 laboratory sexually mature white male rats, which were divided into 3 groups, was investigated by atomic absorption spectral analysis. The 1-st group included 15 intact, practically healthy animals, the 2-nd – 30 rats with one-month diabetic arthropathy of the knee joint, the 3-rd – 30 animals with the two-month specifi ed pathology. Histological micropreparations were also made from the femur and tibia. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin manufactured by «Sigma» at a dose of 50 mg/kg. Quantitative indicators were processed statistically. The content of iron, copper, zinc, magnesium, manganese, lead in the bone tissue of the femur and tibia in case of diabetic arthropathy of the knee joint is disproportionately and unevenly reduced. The iron content in the femur decreased by 3.4% in one-month diabetic arthropathy, in the tibia by 4.9%, and in two-month arthropathy the studied indicators changed by 8.2% and 13.7%, respectively (р<0.001). The content of copper in the monthly diabetic arthropathy of the knee joint in the femur decreased by 4.7%, in the tibia – by 6.8%, with a two-month course of simulated pathology – by 9.3% and 12.7%, respectively (р<0.001). The content of zinc, magnesium, manganese, lead in femur and tibia decreased similarly under the studied experimental conditions. Changes in the content of the studied microelements depend on the duration of diabetic arthropathy and dominate in the tibia. Diabetic arthropathy of the knee joint leads to an uneven, disproportionate decrease in the content of trace elements in the femur and tibia. The most pronounced imbalance between the content of the investigated microelements was found in two-month diabetic arthropathy of the knee joint and in the tibia.