Changes of iodothyronine deiodinase type 1 and type 3 mRNA level and activity in liver of mice with endoto-xemia

Abstract

Objective To explore the effect of endotoxemia on triiodothyronine(T3) and thyroxine(T4), and the level and activity of iodothyronine deiodinase type 1 and type 3 mRNA. Methods Sixteen mice were randomly divided into control group and lipopolysaccharide(LPS) group, with 8 mice in each group. The mouse model of endo-toxemia was replicated in the LPS group. In the both groups, blood samples from femoral week were collected to assay T3 and T4 levels, and the livers were sampled to inspect D1 and D3 mRNA levels and activities. Serum T3 and T4 levels were assayed with radioimmunoassay, D1 and D3 mRNA levels in liver were detected with real-time polymerase chain reaction, the activity of D1 and D3 in liver were measured by using ion-exchange chromatography combined with immunoassay. The data were statistically analyzed by SPSS 13.0 software. Results Statistical differences of T3, D1 and D3 mRNA levels and activities between the 2 groups were found (all P 0.05). Conclusions It is possible that euthyroid sick syndromes happens in endotoxemia episodes, and the changes of D1 and D3 mRNA levels and activities are the possible influencing factors. Key words: Endotoxemia; Iodothyronine deiodinase typeⅠ; Iodothyronine deiodinase type Ⅲ; Euthyroid sick syndromes