Changes of glucose metabolites in gastric tissue and serum in patients with gastric cancer

Abstract

Objective To explore the changes of glucose metabolism products in gastric tissue and serum in patients with gastric cancer.Methods The metabolism products of glucose in gastric tissue and serum were determined using gas chromatography/mass spectrometry.The correlation between glucose metabolites in gastric tissue and those in serum was analyzed.Results Five glucose metabolites including succinate,fumaric acid,L-(+)-lactic acid,α-ketoglutaric acid,and D-glucose were found in the gastric tissue.The expressions of fumaric acid,L-(+)-lactic acid,and α-ketoglutaric acid were (0.332 ±0.136)％ vs (0.276 ± 0.095)％,(0.606±0.254)％ vs (0.408±0.184)％,(0.856±0.583)％ vs (0.534±0.421)％,respectively,in gastric cancer tissue and normal gastric mucosa tissue (P =0.043,P =0.033,P =0.022,respectively).Succinate and D-glucose showed no significant differences between these two tissues (both P ＞ 0.05).Six glucose metabolites including L-(+)-lactic acid,fumaric acid,2-oxygen-methyl-D-arabinose,D-glucapyranoside,D-fructopyranose,and D-glucose were found in the serum.The expressions of fumaric acid and 2-oxygen-methyl-D-arabinose were significantly lower in gastric cancer group than in healthy control group [(0.165 ±0.329)％ vs (0.307 ±0.205)％,(0.152 ±0.172)％ vs (0.250 ±0.217)％ ; P =0.030,P =0.049,respectively].The remaining four metabolites showed no significant difference (all P ＞ 0.05).L-(+)-lactic acid,fumaric acid,and D-glucose were detected in both the gastric tissue and serum.Correlation analysis showed that none of them was associated between the gastric cancer tissue and serum with the values as L-(+)-lactic acid (r=-0.095,P=0.617),fumaric acid (r =-0.056,P =0.768),and D-glucose (r =-0.088,P =0.725).Conclusion Glucose metabolites change in the gastric tissue and serum in patients with gastric cancer; however,they are not associated. Key words: Gastric cancer; Gastric mucosa; Serum; Glucose metabolism