Changes of calcium channel characteristics and its relationship with pancreatic islet β cell parasecretion in rat born small for gestational age

Abstract

Method The animal model of small for gestation in rat was made under matemal calorie restriction by 50% from d1 of gestation until term, while the control groups named AGA rats, were obtained from normal pregnant rats. Both of SGA and AGA newborn rats were fed normally till 4 weeks and 8 weeks. Single islet b cell isolation and pancreatic tissue-slice technique were used to obtain b cells in neonatal rats and adult rats, respectively. Using whole cell patch clamp to record the characteristics of b cell voltage dependent calcium channel (such as current, reversal potential, I-V curve, activation curve, inactivation curve) in both SGA and AGA groups. Using capacitance measurement induced by a sequence of sine wave stimulus to reflect the insu lin secretion. In aw ord, study the tendency of calcium channel kinetics and secretion property by timing. Results The membrane capacitance of SGA was smaller than AGA rat after excluding the influence of whole cell membrane capacitance deviation both in neonatal rats, 4 weeks and 8 weeks (P 0.05). The same result was obtained in activation curve and inactivation curve of calcium channel, whose half activated voltage/ half inactivated voltage V1/2 and slope factor k between SGA and AGA both showed no significant difference (P>0.05) ; 2) adult rats: there was no statistical difference found in all average calcium current density (Id), including the total calcium, L-type and T-type. No significant difference was observed in the peak current density of I-V curve in SGA and AGA (P>0.05). However, the reversal potential in SGA group was much lower than control (P<0.05). The two group had the same shape of activation curve and inactivation curve of calcium channel, and 50% activated voltage between SGA and AGA had no significant difference, but the calcium channel of SGA was easily to be closed, the inactivated voltage in SGA group was much higher than control (P<0.01). Conclusion The effect of intrauterine malnourishment on pancreatic isletbcell last in postnatal life and the destroy of bcell tends to aggravate with time. Low calcium current density on b cell membrane devotes to the decrease of b cell secretion in SGA neonatal rat. However, the current of calcium channel returns to normal level in childhood. The kinetics of calcium channel is changed significantly,