Changes of aspartate aminotransferase and alanine aminotransferase activity in vitamin B6 deficient rat.

Abstract

SUMMARY The aspartate aminotransferase and alanine aminotransfer ase activities in vitamin B6 deficient rats were assayed. The activities of aspartate arninotransferase and alanine arninotransferas e were reduced in the supernatant fraction ， while their activities were preserved in the rnitochondrial fraction. With the addition of pyrido xal phosphate in vitro to the liver or kidney of vit arnin B6 deficient rats ， the supernatant aspartate arninotransferas e recovered its activity to a respectable degree ， while alanine aminotransferase not 80 rnuch. Th e addition of pyridoxal phosphate in vitro had no infiuence upon the of rnitochondrial aspartate aminotransferase and ala Il ine aminotransf erase . The results of these experirnents indicate that these supernat ant transarninases are a狂ected by the diminution of vitamin B6 content .