Abstract
ABSTRACT Using the vibrating probe technique, we have measured transcellular ionic currents ranging from 0.05 to 3.00 μA cm-2 around polar-lobe-forming embryos of Bithynia tentaculata. During maturation and the first two mitotic division cycles, we have detected recurrent changes in the current pattern, which correlated temporally and spatially with cytokinesis and polar lobe formation. The maximum inward current at the animal pole coincides with the onset of cleavages at that pole, and the maximum inward current at the vegetal pole slightly precedes the formation of the two meiotic polar lobes and the first mitotic polar lobe at that pole. This correlation was less clear with regard to the second mitotic polar lobe. The resorption of the polar lobe correlates with a reversal of the current from inward to outward at the vegetal pole. After the resorption of the first mitotic polar lobe, the transmembrane ion flow in the membrane area, which formerly covered the lobe, is different from the surrounding membrane.
Published Version
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