Changes in the spectral pattern of selenium accumulation in Coleus blumei and the effects of chelation.

Abstract

Chemically enhanced phytoremediation has been proposed as an effective approach to remove heavy metals from contaminated soil through the use of high biomass production plants. This study investigated changes in the spectral pattern of selenium (Se) accumulation in Coleus blumei Benth. (coleus) plants grown in hydroponics with 1.0mg/l sodium selenite (Na2SeO3) and the effects of (S,S)-ethylenediamine disuccinic acid (EDDS) thereon through X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDXS) and Fourier transform infrared (FTIR) spectroscopy analyses. When EDDS concentrations were in the range of 0-1.0mmol/l, Se content increased significantly; however, at EDDS concentrations above this range, the symptoms of Se toxicity were alleviated in coleus leaves. Application of EDDS over 1.0mmol/l significantly decreased total Se uptake in the leaves and roots of the plants. The powder diffraction patterns of the roots and leaves displayed sharp crystalline peaks, which were characteristic of an organic molecule with crystallinity. Our results revealed the presence of high amounts of C, O, Mg, Al, Si, K and Ca in the roots and leaves under Se-induced stress with different concentrations of EDDS. There were no changes in the chemical compositions of the roots and leaves, but the contents were influenced by Se-induced stress and EDDS treatment. This study demonstrated the importance of applying XRD, EDXS and FTIR methods toward a more comprehensive understanding of the mechanisms of EDDS-induced Se accumulation in plants.