Changes in the morphometric and cytological indices of the spleen after acute blood loss followed by stem cell injection

Abstract

The goal of the work was to study morphometric and cytological indices of the spleen in mature and old laboratory animals after acute blood loss followed by allogenic transplantation of placenta multipotent mesenchymal stromal cell and hematopoietic stem cells. The study demonstrated the reduction of lymphoid follicles area due to a reduction in the thymus-independent area 5 days after acute blood loss followed by stem cells transplantation to the mature animals. There was no immunosuppressive effect to the white pulp of the spleen in old animals. An increase in leukocytes and erythroid elements was noted in the red pulp of mature animals. In old animals, the increase in cell density in red pulp was mainly due to increased number of erythroid cells.