Abstract
This works studies the physiological characteristics (sperm motility characteristics) and biochemical characteristics (DNA damage, enzymes activities and fertilization ability) of fresh and freeze-thawed semen of sex-reversed female mandarin fish Siniperca chuatsi (n = 30) obtained with the application of 17α-methyl testosterone for sex reversal. The fresh sperm motility and fertilization rate of sex-reversed females were about 83% and 70% respectively which had no significant difference with normal males (p > 0.05). Except for the value of DNA damage, other values of sperm motility, related enzymes activities and fertilization rate of sex-reversed female sperm declined after a process of cryopreservation (p < 0.05). But the frozen sperm can still get nearly 60% of the fertilization rate. This study identified the physiological and biochemical characteristics of the fresh and cryopreserved sperm from sex-reversed female mandarin fish, and the sex-reversed female spermatozoa can be used for actual production.
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