Abstract
To investigate soluble sugar metabolism and its relationship to chilling injury (CI), loquat fruits were stored at 0 and 5°C for 5weeks. CI symptoms were monitored during storage, and soluble sugar content and related enzyme activities were measured. Enzymes activities and substrate levels in the ascorbate-glutathione (AsA-GSH) cycle were also determined. Fruit at 0°C had lower sucrose levels and higher levels of glucose and fructose than fruit maintained at 5°C, and also manifested less severe CI symptoms. Fruit at 0°C also exhibited increased activities of acid invertase, neutral invertase, sucrose phosphatesynthase and sucrose synthase. AsA-GSH cycle activity at 0°C was much higher than that at 5°C. Glucose levels correlated positively with levels of AsA and GSH and ascorbate peroxidase (APX) activity. The increased glucose levels are sufficient to account for the chilling tolerance of loquat fruit.
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