Abstract
The changes in protein profiles of bean leaves (Phaseolus vulgaris) during the infection process of geminivirus, bean golden mosaic virus (BGMV), were studied using one- and two-dimensional polyacrylamide gel electrophoresis. After Coomassie Blue staining, no differences between BGMV- and mock-infected plants were observed in either one- or two-dimensional gel profiles. However, comparison of the protein profiles after incorporation of [35S] methionine showed that two specific polypeptides were induced by virus infection. One was the coat protein of BGMV and the other was a 24kDa protein assumed to be expressed by the host plant genome. During the progress of viral infection, the virus coat protein was strongly expressed at the late stage, while the 24kDa protein was expressed mainly at the early stage of infection. We suggest that this 24kDa protein plays an important role in BGMV infection.
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