Abstract
Carbon-13 nuclear magnetic resonance (NMR) spectroscopy was used in the direct comparison of acetate metabolism in suspension cultures of Datura stramonium, an alkaloid producer in vitro, and Nicotiana phumbaginifolia, a non-producer. Growth of cells in the presence of 4 mM [2- 13C] acetate, resulted in a considerably slower uptake of the substrate from medium in D. stramonium than in N. plumbaginifolia. Initial labeling, primarily of the intracellular glutamine and malate pools was observed in N. plumbaginifolia, and putrescine and GABA pools in D. stramonium. The high activity of glutamine synthetase observed in N. plumbaginifolia, which can lead to the non-synthesis of intracellular putrescine by metabolic deviation of glutamate, would be a first explanation for non-biosynthesis of alkaloids for these cell suspensions. On the contrary, the observation of putrescine labeling is in agreement with alkaloid biosynthesis through D. stramonium cell cultures.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
