Changes in Potential Pathogens During Temperature-controlled Pig Manure Composting Process

Abstract

With the development of intensive livestock and poultry breeding, problems associated with pathogenic microorganisms in animal manure are becoming more obvious. The pathogenic bacteria in pig manure may cause zoonotic transmission, which not only hinder the healthy development of livestock and poultry industry, but also harm people's health. In this study, the diversity and abundance of potential pathogens in the composting process of pig manure under three temperature-controlled treatments (high-temperature group, medium-temperature group and low-temperature group) were analyzed based on high-throughput sequencing technology. A total of 13 potential pathogenic bacteria were identified in the pig manure composting process, among which Enterococcus faecalis and Agrobacterium vitis accounted for the largest proportions (12%). Under different temperature-controlled treatments, the abundances of Pseudomonas stutzeri and Acinetobacter baumannii changed most obviously. Moreover, the diversity and abundance of potential pathogens under high-temperature treatment were significantly lower than those under medium-temperature and low-temperature treatment, indicating that high temperature condition of composting could effectively remove pathogenic bacteria and inhibit the growth and reproduction of pathogens.