Changes in plasma volume and protein content during exposures of working men to various temperatures before and after acclimatization to heat: separation of the roles of cutaneous and skeletal muscle circulation.

Abstract

1. Ten male subjects were trained in stair stepping for 2 weeks. Group A (six subjects) was thereupon sequentially exposed for 45 min to dry bulb temperatures of 20, 40 and 30 degrees C, a vapour pressure of 10-11 mm Hg and a wind speed of 1 m/sec in a climate tunnel. While temperature changes were being effected the subjects rested in an antechamber. Group B (four subjects) was exposed to a sequence of 40, 20 and 30 degrees C. Work rate was the same for all subjects, i.e. 216 kg m/min ( approximately to an oxygen consumption of 0.9 l./min). Duplicate experiments were run on both groups of subjects before and after acclimatization to heat.2. Throughout, periodic samples of venous blood, water and protein movement into or out of the extravascular compartment was assessed during exercise periods wherein blood flow was increased to exercising muscles (Group A, 20 degrees C) or to both exercising muscles and skin (Group B, 40 degrees C; Groups A and B, 30 degrees C.)3. Mild exercise in a cool environment before and after acclimatization to heat was accompanied by expansion of the vascular volume and an increase in the amount of circulating protein.4. Mild exercise in a warm environment for 45 min was accompanied by haemoconcentration and loss of protein from the vascular volume before subjects were heat acclimatized. The results were reversed following heat acclimatization; i.e. exposure of Group B to 40 degrees C and of Groups A and B to 30 degrees C was accompanied by haemodilution and addition to (or maintenance of) plasma protein concentration.5. Effects of heat acclimatization on exposure of Group A to 40 degrees C were also noted.6. The effects of heat acclimatization were ascribed to:(a) a change in permeability of cutaneous capillaries to large molecules,(b) an increased availability of translocatable protein within cutaneous interstitial spaces, and(c) a combination of both a and b.7. Further, the results supported a previous suggestion that addition or loss of water and protein from the vascular volume is dependent on the ratio of cutaneous to muscle blood flow.